Research: Under general supervision provide technical support and carry out most experiments independently in a molecular biology laboratory. Provide complex technical lab support by performing a broad range of complex laboratory and research related tasks including novel nascent RNA sequencing and combined metabolic RNA labeling-sequencing. Perform RNA manipulations using extractions from mammalian/human cells or other tissue samples. Perform DNA quantification, gel electrophoresis, PCR, cloning, quantitative PCR. Perform complex molecular experiments such as high throughput preparation of multiple RNA samples, single cell samples and next-generation sequencing.
Lab duties: Responsible for day-to-day laboratory duties; organize laboratory workflow, receive and maintain database of supplies. Develop and maintain complex experimental and computational laboratory records. Provide overall maintenance of the laboratory; clean lab ware, stock media and order lab supplies, manage laboratory waste streams, and assist in keeping the lab orderly. Prepare moderately complex stains, media, buffers, and reagents, such as chemical RNA modification reagents.
Shipping process : Process and handle all incoming sample shipments. Unpack frozen or dry samples and ensure proper storage by transferring to freezers or dry storage areas. Update and maintain sample databases and records. Organize sample collections and RNA extracts in freezers. Handle the packaging and shipping of samples and materials to/from other laboratories, via various carriers. Requires training for dry ice shipping.
Safety requirements : Adhere to lab safety protocols with a solid understanding of laboratory safety techniques and procedures. Ensure the proper disposal of laboratory waste and hazardous materials. Follow direction and training as needed.
Training and professional development : Pursue individual professional development as determined jointly with supervisor. As directed, attend seminars and training to promote and communicate expertise and knowledge in lab. Attend training and expansion of skills through participation in job-related courses, seminars and workshops.
Other duties as assigned