#x 9; Design and implement strategies for protein mutagenesis to enhance or modify protein function. Perform site-directed mutagenesis and other molecular biology techniques to introduce mutations. Optimize protein expression in various systems such as E. coli, yeast, or mammalian cells. Purify and characterize proteins using techniques such as chromatography, SDS-PAGE, and mass spectrometry. Conduct high-throughput screening of protein variants to identify those with desired properties. Analyze... more details
Protein Engineering Research Associate Kelly® Science & Clinical is seeking a Scientist for a direct hire position at an early-stage biotech company in Hayward, CA. If you’re passionate about bringing the latest scientific discoveries to life and are ready to take the next step in your career, trust The Experts at Hiring Experts.
Salary: $80-100k
Schedule: Monday-Friday, standard working hours Responsibilities:
Design and implement strategies for protein mutagenesis to enhance or modify protein function.
Perform site-directed mutagenesis and other molecular biology techniques to introduce mutations.
Optimize protein expression in various systems such as E. coli, yeast, or mammalian cells.
Purify and characterize proteins using techniques such as chromatography, SDS-PAGE, and mass spectrometry.
Conduct high-throughput screening of protein variants to identify those with desired properties.
Analyze protein structure and function to guide the design of future mutations.
Utilize computational tools to model protein structures and predict the impact of mutations.
Develop and perform assays to assess protein stability, activity, and other functional properties.
Collaborate with cross-functional teams to integrate protein engineering efforts into broader project goals.
Document experimental procedures and results meticulously in laboratory notebooks and digital records.
Prepare reports and presentations summarizing findings for internal stakeholders and external collaborators.
Stay current with scientific literature to integrate new methods and technologies into research projects.
Troubleshoot and optimize protocols to improve experimental outcomes.
Collaborate with team members to analyze data, interpret results, and prepare research reports.
Qualifications:
BS/MS degree in a relevant field and a minimum of 2 years of industry experience
Proficient in mammalian cell culture
Proficiency in molecular biology, gene editing, DNA library prep, next-generation sequencing (NGS), qPCR, flow cytometry and analysis
Hands-on experience with site-directed mutagenesis and other molecular cloning techniques.
Proficiency in protein expression systems such as E. coli, yeast, or mammalian cells.
Experience with protein purification techniques, including chromatography and centrifugation.
Knowledge of protein characterization methods such as SDS-PAGE, Western blotting, and mass spectrometry.
Familiarity with high-throughput screening techniques to assess protein properties.
Understanding of protein structure and function and the ability to predict the effects of mutations.
Experience with computational tools for protein modeling and analysis (e.g., PyMOL, Rosetta, or Chimera).
Proficiency in using analytical techniques such as spectroscopy (UV-Vis, fluorescence) and calorimetry.
What happens next: Once you apply, you’ll proceed to next steps if your skills and experience look like a good fit. But don’t worry – even if this position doesn’t work out, you’re still in our network. That means our team of expert Science & Clinical recruiters will have access to your profile, making your opportunities limitless.
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