#x 9; Execute cellular and molecular biology protocols in the laboratory Maintain cancer cell lines and establish new stable lines via lentiviral transduction Validate engineered cell lines by reporter assays and PCR Perform genetic targeting using si. RNA, sh. RNA or CRISPR/ Cas 9 technology for cell line characterization and screening Conduct routine molecular and cellular experiments including g. DNA and RNA isolation, PCR, cloning, western blot analysis, flow cytometry, and microplate cell p... more details
Kelly is hiring for a Research Associate position with one of our clients in San Diego, CA.
- Full time- W2
- 12 months contract (May be extended or converted)
- 100% on-site, lab-based role.
- $ 26.58-$33.75/hr
The Oncology Discovery team at the Oncogenesis Thematic Research Center is seeking a Research Associate to support our drug discovery and characterization efforts.
An early-career level position. Familiarity with core industry and lab practices and standards. Solves routine problems of limited scope and complexity. The qualified candidate will leverage various CRISPR/Cas9 technologies to enable genetic/multiplexed cell line screens to support early and late stage drug mechanism of action studies. They will assist senior team members in performing the totality of the screening process, including cell line engineering, cell treatment experiments, NGS library preparation, and target validation. Common skills and techniques used will include mammalian cell culture, lentiviral transduction, DNA/protein isolation, PCR, flow cytometry, and western blot.
The successful candidate will demonstrate strong verbal and written communication skills and will be capable of working collaboratively in a multidisciplinary environment. Must demonstrate the ability to multi-task and prioritize time management of daily activities to deliver timely results. Wet lab research experience is required.
Job Description:
- Execute cellular and molecular biology protocols in the laboratory
- Maintain cancer cell lines and establish new stable lines via lentiviral transduction
- Validate engineered cell lines by reporter assays and PCR
- Perform genetic targeting using siRNA, shRNA or CRISPR/Cas9 technology for cell line characterization and screening
- Conduct routine molecular and cellular experiments including gDNA and RNA isolation, PCR, cloning, western blot analysis, flow cytometry, and microplate cell proliferation assays
- Identify experimental issues, troubleshoot, and recommend a corrective course of action
- Design experiments and protocol optimization in collaboration with senior researchers
- Maintain details lab notebooks and record data in electronic notebook in accordance with company policy
- Communicate results in written and oral presentations
Qualifications and Education Requirements:
- Bachelor’s degree in Biology
- 2+ years of wet lab experience (not including lab classes): experience in mammalian cell culture is required.
- Basic molecular biology skills (PCR especially; familiarity with library prep preferred)
- Proficient in laboratory math (dilutions, cell count-based plating)
- Excellent organizational skills and record-keeping (Excel & Powerpoint)
Important information: This position is recruited for by a remote Kelly office, not your local Kelly branch. Applicants must be legally permitted to work in the United States. Please apply online to be considered for the position.
As part of our promise to talent, Kelly supports those who work with us through a variety of benefits, perks, and work-related resources. That’s why we’re proud to offer a complete array of voluntary benefits to members of the Kelly talent community.
Apply Today!
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