Our research examines how hormonal signals affect tissue development and function. We are particularly interested in genetic diseases that affect the skeletal system (bone, cartilage, and muscle). We use a combination of human cell models and mouse models to study how genetic changes in hormone signaling affects skeletal formation. Our vibrant translational research program also uses advanced models such as microfluidic “organs on a chip” to assist with disease modeling, as well as human induced pluripotent stem cells, single cell RNA sequencing, and small molecule screening.
The individual hired will have a number of responsibilities related to these overall goals. These include deriving and maintaining human stem cell cultures, maintaining a small mouse colony, handling and genotyping mice, and assisting with experiments. Techniques used will include the analysis of mouse models, in vitro and in vivo skeletal tissue analysis, cell culture, protein analysis, cloning, PCR analysis, single cell sequencing, and standard molecular biology techniques. The individual will also work closely with other members of our team to assist with human tissue sample collection and processing. In addition, the individual will act as a lab manager, assist with establishing and maintaining the overall function of the laboratory (including stocking supplies, ordering, and arranging for maintenance of equipment). The individual will also assist with grant writing and paper writing.
The successful candidate must have excellent communication skills as our work is very much team based. The successful candidate also needs prior laboratory experience with basic molecular biology methods (PCR, protein analysis, standard molecular biology techniques, flow cytometry) and must be highly organized, detail oriented, and a team player. The successful candidate will also assist with ongoing clinical-translational research projects, with basic management of the laboratory’s day to day operations and maintenance of our biobank.
The final salary and offer components are subject to additional approvals based on UC policy.
To see the salary range for this position (we recommend that you make a note of the job code and use that to look up): TCS Non-Academic Titles Search (https://tcs.ucop.edu/non-academic-titles)
Please note: An offer will take into consideration the experience of the final candidate AND the current salary level of individuals working at UCSF in a similar role.
For roles covered by a bargaining unit agreement, there will be specific rules about where a new hire would be placed on the range.
To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html
- Experience with human embryonic stem cell or induced pluripotent stem cell derivation and culture
- Fluorescence microscopy / confocal microscopy experience
- Masters degree or advanced degree relevant to biomedical research
- 1 or more years of recent, directly related biomedical research lab experience
- 1 or more years of experience managing a mouse colony
- 1 or more years of experience manipulating mice in a research setting, and willingness to do so
- Experience dissecting mice
- Experience with IV and IP injection into mouse
- 1 or more years of experience with general cell culture techniques, including maintenance of cell lines, transfection techniques, isolation of RNA and cDNA
- Experience with laboratory management and organization, including ordering, stocking supplies, and coordinating equipment repairs.
- 2 year commitment
SRA 1:
- BA/BS in biomedical research or related science and knowledge of, or experience with, the basic techniques or methods required by the position; and/or an equivalent combination of education and experience;
- Recent (within the past two years) direct laboratory experience using the techniques or methods required by the position.
- Knowledge of laboratory, biohazard and radiation safety is required since the individual will be working in a BSL2* environment with hazardous agents, infectious agents, and radiological materials.
- Knowledge of and experience with PCR and basic molecular biology techniques, including cloning, bacterial cultures/propagation, plasmid isolation
- Formal undergraduate coursework in molecular biology or experience in a molecular biology research laboratory
- Computer skills including the use of Microsoft office programs (Excel and Word), Adobe Photoshop, and sequence analysis programs (Vector NTI or equivalent)
- Careful attention to detail. Maintain a detailed laboratory notebook of experiments done on a daily basis
- Ability to work independently and to assess need for interaction with supervising faculty member
- Strong quantitative skills
- Strong organizational ability and aptitude to follow laboratory standard operating procedures; ability to process and maintain databases to help organize the laboratory.
- Strong oral and written communication skills, including data presentation, the preparation of tables, charts, and graphs, and the fundamentals of statistical analysis
- Demonstrated ability to work as part of a scientific research team
- Managerial skills (basic accounting and stocking experience)
- Ability to work independently and to assess need for interaction with supervising faculty member; knowledge of and experience with lab management, training lab personnel
- Quick learner, shows initiative, works very well with others in a collaborative setting, works very efficiently, can prioritize and multitask
- Basic knowledge of data presentation, including the preparation of tables, charts, graphs and the fundamentals of statistical analysis
SRA 2:
- In addition to the above, one or more years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position and demonstrating technical expertise in these areas; or an equivalent combination of education and experience
- Experience with general cell culture techniques including maintenance of cell lines, transfection techniques, isolation of RNA and cDNA
- Experience with flow cytometry
- Experience with protein biochemistry and immunochemistry including SDS-PAGE, western blotting, ELISA
